Protein_Domain

Part:BBa_J70593:Design

Designed by: Joseph Lynch   Group: Knight Lab   (2010-06-18)

RFC12 ATG Head Domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Made with synthetic oligos: 

5' AATTC GCGGCGC T ACTAGT ATG GCTAGC A GCGGCCG CTGCA 3', Head-F

5' GCGGCCGCTGCTAGC CAT ACTAGTAGCGCCGCG 3', Head-R

Note that both primers were ordered phosphorylated. An alternative is to phosphorylate the primers yourself with a kinase.

Source

Common knowledge.

References